enzyme-linked immunosorbent assay of progesterone in serum using penicillinase as label

Authors

بهرخ فرهمند

behrokh farahmand محمد جواد رسایی

mohamad javad rasaee ناصر ملک نیا

naser maleknia محمد ملکانه

mohamad malekaneh

abstract

an enzyme-linked immunosorbent assay (elisa) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. a c3 and c11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (bsa). polyclonal antibody against these two immunogens were prepared in new zealand white rabbits. purified ig fractions of antibodies were immobilized onto the wells of microtiter plates. progesterone 3-(o-carboxymethyl ) oxime was linked to penicillinase and used as tracer (enzyme-conjugate). the standard assay completed within three hours had a low limit of detection, from 5 pg/well (50 pg/ml) covering up to 1 ng/well (10 ng/ml). for the first time in this assay the color development in case of penicillinase as enzyme label was measured in the wells directly. recoveries were measured to be within the range of 95-98%. percent coefficient of variations (cv%) obtained between and within runs of several assays were 3% and 7% respectively. when compared between values of progesterone measured by radioimmunoassay (ria) and present elisa method, a correlation value of r = 0.9 was obtained. freeze-dried progesterone-enzyme conjugate was found stable for at least three months at 4° c without using any other preservative.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Enzyme-Linked Immunosorbent Assay of Progesterone in Serum Using Penicillinase as Label

An enzyme-linked immunosorbent assay (ELISA) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. A C3 and C11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (BSA). Polyclonal antibody against these two immunogens were prepared in New Zealand white rabbits. Purified Ig fractions of antibodies were immobilized o...

full text

ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL

An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...

full text

enzyme-linked immunosorbent assay of neopterin using penicillinase as label

an enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. immunoglobulin fractions were purified and coated on wells of microtiter plates. a chain heterology was introduced in neopterin derivative and conjugated to penicillinase. the assay is...

full text

DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL

An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...

full text

A Heterologous Enzyme Linked Immunosorbant Assay of Morphine Using Penicillinase as Label

A rapid, sensitive, specific and high through-put enzyme-linked immunosorbant assay (ELISA) methodfor determination of morphine in urine samples using penicillinase as label enzyme has been developed. Noextraction or chromatography was included in this assay procedure. Immunoglobulin (Ig) purified polyclonalanti-bodies against a C6-hemisuccinate derivative of morphine (M-C6-HS...

full text

development of a simple and sensitive enzyme- linked immunosorbent assay (elisa) for clinical measurement of testosterone using penicillinase as label

an enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (t-3-0-cmo-bsa ) and penicillinase-labelled t-3-0-cmo was developed. this assay was utilized to measure testosterone in serum samples of male and female subjects. the sensitivity of the assay is 50pg/well and the antibody developed crossreacted...

full text

My Resources

Save resource for easier access later


Journal title:
iranian biomedical journal

جلد ۲، شماره ۳، صفحات ۱۱۵-۱۲۲

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023